Intracellular O2 Measurements

The Combination of Fluorescence Microscopy and Nanosensors

  • Fig. 1: Changes in intracellular oxygen content upon addition of FCCP (1 µm) and antimycin A (10 µm). 100% air saturation corresponds to 210 µm O2 at 37 °C. The error bars correspond to approximately seven single measurements (1 s each) per data point.Fig. 1: Changes in intracellular oxygen content upon addition of FCCP (1 µm) and antimycin A (10 µm). 100% air saturation corresponds to 210 µm O2 at 37 °C. The error bars correspond to approximately seven single measurements (1 s each) per data point.

The combination of soluble cell-penetrating oxygen sensor and an optoelectronic accessory unit for standard fluorescence microscopes allows for a simple and robust determination of the intracellular oxygen content in cell samples. An advantage of the system is its ability to overcome interfering background signals. The system evaluates the phosphorescence decay time even from weak and interfered signals and relates them to the oxygen concentration. For demonstration, cells were treated with carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) and antimycin A and the corresponding changes in the intracellular oxygen content were recorded.

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Authors

Sebastian Prill1, Anika Andersson2, Dmitri B. Papkovsky3 and Elmar Schmälzlin4

1 Fraunhofer Institute for Biomedical Engineering (IBMT), Dept. Cellular Biotechnology, Potsdam‐Golm, Germany
2 UP Transfer GmbH, Potsdam
3 University College Cork, Biochemistry Department, Cork, Ireland
4 Colibri Photonics GmbH, Potsdam‐Golm

Contact

Colibri Photonics GmbH
Am Mühlenberg 11
14476 Potsdam
Deutschland
Phone: +49 331 95145809
Telefax: +49 331 95145807

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