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You are here: Home › Applications Overview › Characterization of Critical Parameters in Bioprocess Development - Development of a Shaken Scale-Down Model

Characterization of Critical Parameters in Bioprocess Development - Development of a Shaken Scale-Down Model

Jan. 27, 2012
Fig. 1: SFR Shake Flask Reader
Fig. 1: SFR Shake Flask Reader more
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A shaken scale-down model at approx. 0.1 L scale for a previously established stirred-tank model at 2 L scale was developed. In the shaken scale-down model online monitoring of pH and dissolved oxygen concentration was performed using the Shake Flask Reader (SFR) by PreSens. Though only a simple, manual control of these two parameters could be realized the shaken scale-down model achieved similar data to the stirred-tank model for viable cell density, specific glycosylation content, and ion exchange chromatography pattern. These results suggest that for this process the importance of pH and DO control might be overestimated and further investigations have to be made for evaluating and comparing the influence of shear stress and power input in both models.

Authors:
Holger Kley, Roche Diagnostics GmbH
Jan Kreuzmann, Roche Diagnostics GmbH
Caroline Schuster, Roche Diagnostics GmbH
Robert Puskeiler, Roche Diagnostics GmbH

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Keywords : 2 L Scale Model Bioprocess Development Bioprocessing Biotechnology Caroline Schuster Chromatography Critical Parameter Glycosylation Holger Kley IEC pattern Jan Kreuzmann laboratory equipment Life Science pH Pharma Presens product concentration Robert Puskeiler Roche Screening Application Note SFR Shake Flask Reader Shaken Scale-Down Model Shaker

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