PreSens on Growth Control - Non-invasive Measurement of Dissolved Oxygen & pH in Shake Flask Cultures

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PreSens: Shake flasks are the most commonly used cultivation system. A major, though often neglected problem in aerobic shake flask cultivation is limited oxygen supply or an incorrect pH value.

Enhanced Culture Performance
The optimal oxygen supply of cells and perfect pH conditions are essential basic requirements for high quality cell cultures. A shortage in oxygen significantly influences the cellular metabolism; the same applies for shifts of pH. Different factors like the geometry of baffles or the application or production of surface active compounds can change the oxygen mass transfer unpredictably. PreSens' new Shake Flask Reader (SFR) allows parallel optical online measurement of dissolved oxygen concentration (DO) and pH in shake flask.

Read more in this article on the

• Optical Sensor Technology
• Culture Conditions
• Metabolic Events
• Buffer Requirements
• Reliable Screening Results

Authors:
Konstantin Schneider
Verena Schütz
Elmar Heinzle
Biochemical Engineering Institute
Saarland University
Saarbrücken, Germany

Gernot Thomas John
PreSens Precision Sensing GmbH

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Keywords : Batch Cultivation Biological Monitoring Bioprocessing Cell Biology Cell Culture cellular metabolism dissolved oxygen concentration Elmar heinzle Gernot Thomas John Growth Control Konstantin Schneider Monitoring Non-invasive Measurement Online Monitoring optical online measurement oxygen supply pH Presens Saarland University Screening Sensor technology SFR Shake Flask Reader Shaker Surface Active Compound Verena Schütz

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Josef-Engert-Str. 11
93053 Regensburg
Germany

Tel: +49 941 942 72 0
Fax: +49 941 942 72 27
Web: http://www.presens.de

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