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Ultra Performance Supercritical Fluid Chromatography (UPSFC)

Aug. 19, 2011
Fig. 1: van Deemter curves using the UPSFC system for different particle size columns. At each tested flow rate, the chromatographic efficiency increases as particle size decreases.
Fig. 1: van Deemter curves using the UPSFC system for different particle size columns. At each ... more
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Supercritical fluid chromatography (SFC) is a chromatographic technique that employs a supercritical fluid, most commonly CO2, combined with organic co-solvents, such as methanol and ethanol, as the mobile phase. The low viscosity and high diffusivity of supercritical fluids offers fast separations with high chromatographic efficiency.

Introduction

Since supercritical CO2 has properties similar to alkane hydrocarbons, and SFC is often performed on polar stationary phases, it is generally considered a normal phase chromatographic technique [1] complimentary to reverse phase HPLC in selectivity. Spurred by an increased emphasis on chirality in drug discovery and global initiatives on green chemistry across the pharmaceutical industry, analytical SFC is replacing normal phase HPLC as the preferred technique for chiral and achiral separations [2]. Until recently, analytical SFC instruments were primarily used as scouting systems to identify optimal mobile phases and stationary phases for ensuing preparative SFC runs. Largely based on older HPLC technology, these analytical SFC instruments typically suffice as scouting stations, and are not nearly as robust and reliable a technology as HPLC. Historical issues with analytical SFC instruments [2] include: large system volumes that prevent the adoption of small chromatographic particles and high throughput analysis, unstable co-solvent delivery at...

Authors:
Rui Chen, Waters Corporation, New Castle, DE, USA
Andrew Aubin, Waters Corporation, Milford, MA, USA

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Keywords : Andrew Aubin Chromatography Rui Chen Separation SFC Supercritical Fluid Chromatography Ultra Performance Supercritical Fluid Chromatography UPSFC

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