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Super-resolution Optical Microscopy Technology

3D Imaging of Small Structures inside Cells

Jul. 26, 2010
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Fig. 1: Comparison of conventional and STORM images of mitochondria in a mammalian cell. The mitochondrial outer membrane protein Tom20 was labeled. (left panel) Conventional image of the left part of the cell. (middle panel) 3D STORM image of the middle part of the cell. The z-dimension information is color-coded according to the color scale bar. (right panel) The xy cross-section of the STORM image of the right part of the cell. Image courtesy of Zhuang Research Group, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA.
Fig. 1: Comparison of conventional and STORM images of mitochondria in a mammalian cell. The ... more

Fluorescence microscopy is among the most widely used imaging methods in biology. However, conventional light microscopy cannot resolve many structures inside cells because its horizontal resolution is limited to approximately 200 nm. 3D STORM is a recently developed super-resolution technique that can achieve 20-30 nm lateral resolution and 50-60 nm axial resolution, enabling elucidation of previously unresolvable sub-cellular structure. Expansion to multiple fluorophores provides the specificity to interrogate individual biomolecular interactions and to test previous assumptions of co-localization.

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Keywords : 3D Fluorescence imaging Microscopy Nikon STORM

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