Automation of Protein Sample Preparation by Perfinity Workstation

Perfinity Workstation by Shimadzu more

Scientists know that the most important part of mass spec analysis is the preceding separation steps. That is why Perfinity integrated their proprietary automation and column technologies into Shimadzu instrumentation, resulting in the Perfinity Workstation, a multicolumn apparatus that automates protein separations and mass spec sample preparation for more efficient LC-MS analysis.

The workstation is powered by Shimadzu hardware components that work in conjunction with five Perfinity Optimized Columns. Each column performs a step of the mass spec sample preparation process - affinity selection, buffer exchange, digestion, desalting and reverse-phase separation. The automated integration of these steps enables users to start with serum and have peptides ready for LC-MS analysis in as little as 10 minutes.

The workstation combines the selectivity of antibodies with the resolving power of chromatography. Buffer exchange and desalting are performed on-line. The reduction in sample processing time enables users to screen a variety of conditions rapidly.

The major advantage of the Perfinity Workstation's sample preparation approach is that users are not required to immobilize antibodies. Instead, scientists can add antibodies directly to the sample.

This platform is customizable across application areas, including assay development, protein purification, drug development and biomarker discovery. For example, methods built into the workstation automate core steps of assay development, limiting the amount of protocol optimization required.

Related Articles :

• G.I.T. InnovationsAward 2012 - The Final!
• The European Lab Automation Conference and Exhibition 2012
• Partition Chromatography HPCCC
• Spider Silk Proteins: Structure and Function Revealed by NMR Spectroscopy
• Thin Layer Chromatography (TLC) - Online and Offline Coupling with Mass Spectrometry

Keywords : Assay Automation Biomarker Chromatography Drug Development LC-MS liquid chromatography Mass Spectrometry Pittcon 2011 Pittcon 2011 Product Protein Purification Proteomics sample preparation Separation Shimadzu

Shimadzu Europa GmbH
Albert-Hahn-Strasse 6-10
47269 Duisburg
Germany

Tel: + 49 203 76870
Fax: + 49 203 766625
Web: http://www.shimadzu.eu

• Legal
  • Terms and Conditions
  • Privacy Policy
  • Imprint
• Help
  • FAQ
  • Sitemap
  • Contact
• Participate
  • Register
  • Login
  • RSS-Feeds
• Related Publications
  • G.I.T. Imaging & Microscopy
  • CHEManager
  • CHEManager Europe
  • PRO-4-PRO