Products
Efficient Targeted Integration into Mouse Rosa26 Locus with Zinc Finger Nucleases
Knock-In Any Gene without Transgene Landing Pads
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Sigma Life Science launched the CompoZr Targeted Integration Kit - mRosa26, which can precisely and rapidly integrate transgenes into the mouse genome at the Rosa26 safe harbor locus. This kit follows the introduction of the CompoZr Targeted Integration Kit - AAVS1, for transgene integration in the human genome.
Traditional transgenic approaches rely on random integration of a transgene. This low efficiency approach results in multiple random integration events at off-target insertion sites, and often requires the use of antibiotic selection strategies. In addition, the integration event can involve multiple copies of the transgene. Using the CompoZr ZFN technology, a single copy of a transgene can be targeted and inserted in the Rosa26 locus, which is a well-defined safe harbor site in the mouse genome.
Sigma's CompoZr Targeted Integration Kits use zinc finger nucleases (ZFNs) for precise and heritable gene knockin at the target locus (Rosa26 or AAVS1). Both Human and Mouse kits are supplied with the necessary ZFNs and donor plasmid to successfully perform transgene integration by ZFN-assisted homologous recombination, reducing the timescale and complexity of engineering transgenic mouse and human cell lines.
Features and Benefits
Effective Integration Efficiency
• Any mouse cell type can be modified with the CompoZr Targeted Integration Kit - mRosa26
• The ZFNs are a well-validated pair that cleaves the target DNA with high specificity
• ZFN mediated targeted integration can be achieved with high success rates
No Transgene Landing Pads Required
• Prior to ZFN transfection cell lines don′t require engineering of transgene landing pads
• Lack of need for transgene landing pads enables immediate transfection of cell lines with GOI
Rapid Biallelic Integrations with a Single Transfection
• Monoallelic and biallelic integrations have been generated with ZFNs
• Gene integrations can occur in as little as 48 hours
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Keywords : AAVS1 CompoZr Targeted Integration Kit genomics Kit Knock-In Molecular Biology Rosa26 Sigma Zinc Finger
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