Intracellular O2 Measurements
The Combination of Fluorescence Microscopy and Nanosensors
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The combination of soluble cell-penetrating oxygen sensor and an optoelectronic accessory unit for standard fluorescence microscopes allows for a simple and robust determination of the intracellular oxygen content in cell samples. An advantage of the system is its ability to overcome interfering background signals. The system evaluates the phosphorescence decay time even from weak and interfered signals and relates them to the oxygen concentration. For demonstration, cells were treated with carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) and antimycin A and the corresponding changes in the intracellular oxygen content were recorded.
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Sebastian Prill1, Anika Andersson2, Dmitri B. Papkovsky3 and Elmar Schmälzlin4
1 Fraunhofer Institute for Biomedical Engineering (IBMT), Dept. Cellular Biotechnology, Potsdam‐Golm, Germany
2 UP Transfer GmbH, Potsdam
3 University College Cork, Biochemistry Department, Cork, Ireland
4 Colibri Photonics GmbH, Potsdam‐Golm