Cytotoxicity Determination: A Method for the Evaluation of New Active Pharmaceutical Ingredients
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With an increasing number of new active pharmaceutical ingredients, it becomes more and more important to find efficient and fast screening approaches for industrial application. The objective of this project was to develop a cytotoxicity assay with high throughput by monitoring the cellular respiration in cell culture.
When cells are treated with cytotoxic compounds, they tend to become apoptotic and do not consume anymore oxygen . If the amount of oxygen diffusion into the medium is greater than the oxygen consumption of the remaining cells that survived, the overall dissolved oxygen content in the medium increases. In contrast to this, when treating cells with cytostatic compounds, the oxygen consumption should stay relatively constant as a result of stopping the proliferating process.
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