Cytotoxicity Determination: A Method for the Evaluation of New Active Pharmaceutical Ingredients

  • Fig. 1: Online monitoring of oxygen content in medium caused by cellular respiration of CHO-K1 cells before and during treatment with lethal and sub-lethal concentrations of malonic acid. Shown are the average values based on measurements collected from 3 equal wells (n=3) over time.Fig. 1: Online monitoring of oxygen content in medium caused by cellular respiration of CHO-K1 cells before and during treatment with lethal and sub-lethal concentrations of malonic acid. Shown are the average values based on measurements collected from 3 equal wells (n=3) over time.

With an increasing number of new active pharmaceutical ingredients, it ­becomes more and more important to find efficient and fast screening ­approaches for industrial application. The objective of this project was to ­develop a cytotoxicity assay with high throughput by monitoring the cellular respiration in cell culture.

When cells are treated with cytotoxic compounds, they tend to become apoptotic and do not consume anymore oxygen [1]. If the amount of oxygen diffusion into the medium is greater than the oxygen consumption of the remaining cells that survived, the overall dissolved oxygen content in the medium increases. In contrast to this, when treating cells with cytostatic compounds, the oxygen consumption should stay relatively constant as a result of stopping the proliferating process.

Read more within the full pdf.

Authors

Contact

PreSens GmbH
Am BioPark 11
93053 Regensburg
Germany
Phone: +49 941 942 72 0
Telefax: +49 941 942 72 27

Register now!

The latest information directly via newsletter.

To prevent automated spam submissions leave this field empty.