Ultra Pure Water in Cell Cultivation
- Fig. 1: Arium pro UF ultrapure water system, © Sartorius
- Fig. 2: Schematic drawing of the Arium pro UF ultrapure water system.
- Fig. 3a: Growth curves of PER.C6 EpCAM cell lines in T-flasks, Fig. 3b: Growth curves of PER.C6 EpCAM cell lines in spinner flasks
- Fig. 4: Antibody production in cells cultivated in media reconstituted with UF water (Mab Arium), ready-made media (Mab Control) and RO water (Mab RO) in spinner flasks
- Table 1: Typical mains water impurities and target values for cell culture work .
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Water is a major component of all cell culture media and is therefore needed to prepare media, buffers, and additives, and as well as to serve many ancillary functions, such as heating, cooling, cleaning and rinsing. Thus, water quality plays an important role in the outcome of cell culture experiments.
Contaminants in water used for cell cultures can occur in many forms, such as bacteria, yeasts or molds. These contaminants are usually visible to the eye or by optical microscopy. However, contamination from chemicals or other biological agents may also affect growth, morphology or behavior of cultured cells, yet be undetectable to the unaided eye. Water used in cell cultures must therefore be free of microorganisms and, in particular, of endotoxins, inorganic ions (heavy metals such as lead, zinc, etc.), and organic compounds (humic acids, tannins, pesticides, etc.). For more detailed information, please refer to the reference literature [1, 2]. Examples of typical impurities in mains water (tap water) and target values for cell culture work are shown in table 1.
The objective of the present test series was to evaluate whether ultra pure water produced by Arium pro UF can be readily used for cell culture applications without entailing any problems. In this study, we cultivated PER.C6 EpCAM cells in ready-made CDM4PERMab (Hyclone) media employed as controls, as well as in CDM4PERMab (Hyclone) powder media prepared using ultrapure water obtained with Arium pro UF (UF water) and RO water, respectively, for test purposes. Data for RO water in this Application Note were obtained using the predecessor model (Arium RO) of the current Arium advance system. The results of each culture were then used to assess whether the water produced by Arium pro UF is suitable for use in the cultivation of PER.C6 EpCAM cells.
The PER.C6 cell line derived from human retinoblast cells described and employed in our test series is also used today for the expression of recombinant proteins and monoclonal antibodies (Mab production) and for the manufacture of therapeutic proteins and monoclonal antibodies.
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