Measurements Across Multiple Cell Lines
Cell Count & Viability Assay
- Read Whitepaper
The assessment of cell concentration in combination with viability is an important step in the characterization of cell health. Current methods rely on multiple, sometimes complex, instrument platforms to provide these answers, reducing flexibility, limiting the ability to simply obtain comprehensive cell health information and adding increased costs to researchers.
Other, simpler methods provide inconsistent results due to their dependence upon single-uptake dyes, which do not effectively discriminate between the various states of cellular demise.
The Muse Cell Analyzer enables multidimensional cell health analysis on a single platform. This benchtop cell analyzer guides users through the acquisition and analysis of samples using mix-and-read assays with an intuitive touchscreen interface which delivers rapid measurements of cell concentration, viability, apoptotic status, and cell cycle distribution. Using multiparametric fluorescent detection of individual cells via microcapillary flow technology, the system enables highly sensitive and rapid detection of cellular samples using minimal cell numbers.
The Muse Count & Viability Assay is a simple, rapid, linear assay that provides cell concentration and viability information. In this article, we show that the assay provides superior performance to conventional viability and count measurement by Trypan blue exclusion.
The assay utilizes a proprietary mix of two DNA intercalating fluorescent dyes in a single reagent. One of the dyes is membrane permeant and will stain all cells with a nucleus. The second dye only stains cells whose membranes have been compromised and are dying or dead. This combination allows for the discrimination of nucleated cells from those without a nucleus or debris, and live cells from dead or dying resulting in both accurate cell concentration and viability results. Stained samples are then analyzed using a guided touch screen user interface. The assay display results in a results page with an optional plot display.
The use of dual fluorescent probes that clearly identify all nucleated cells, live and dead, allows for greater sensitivity and accuracy compared to colorimetric methods.
Read more on the assay sample preparation within the full pdf.