Challenges in Quantitative Bioanalysis
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High selectivity is a key component of successful quantitative bioanalysis. The ever increasing sensitivity and throughput requirements of bioanalytical assays often pose method development challenges. A new technology in ion mobility brings the power of differential ion mobility separation to complex mass spectrometric bioanalysis.
One of the most common issues is the presence of a matrix interference which must be eliminated by adjusting HPLC conditions, or modifying sample preparation. Interferences can be present as an un-resolved chromatographic peak, or as a high baseline. In some cases, separation of isomers is required. If a high baseline problem cannot be solved, LOQ's and dynamic range are adversely impacted. Resolving a difficult chromatographic interference can require slower chromatography or more complicated and labor intensive sample clean-up. It also slows down data review if peak integration has to be manually adjusted on a sample by sample basis. Ion mobility presents an attractive option in increasing MS/MS selectivity by providing additional selectivity during sample introduction, following atmospheric pressure ionization. Although ion mobility techniques have been used extensively for qualitative applications, they have traditionally lacked the required ruggedness and speed required for quantitative bioanalysis. The new SelexION technology exhibits several technological innovations that dramatically increase selectivity separation capacity in ion mobility mass spectrometry.