Autophagosomal Membrane Located
Autophagy: The location of the site where autophagosomal membranes are created within the cell has been the subject of two competing models for many years. Now, a group of researchers from Osaka University led by Prof. Maho Hamasaki has located the origin of autophagosomes within the cell and shown that neither model is correct.
Writing in Nature (link to the article), they show that autophagosomes form at the contact site of the endoplasmic reticulum (ER) with mitochondria.
Live cell imaging studies, using an Andor Revolution XD spinning disk confocal microscope equipped with three Andor iXon 3 EMCCD cameras, captured previously hidden detail. The research revealed the re-localisation of the pre-autophagosome/autophagosome marker ATG14 to the ER-mitochondria contact site after starvation, along with the complementary localisation of the autophagosome-formation marker ATG5 until formation is complete.
To view videos and figures of the original article, please click on the following links:
Maho Hamasaki, Nobumichi Furuta, Atsushi Matsuda, Akiko Nezu, Akitsugu Yamamoto, Naonobu Fujita, Hiroko Oomori, Takeshi Noda, Tokuko Haraguchi, Yasushi Hiraoka, Atsuo Amano & Tamotsu Yoshimori. "Autophagosomes form at ER-mitochondria contact sites," Nature 495, 389-93 (2013) | doi: 10.1038/nature11910.