Trap & Identify Molecules at Trace Concentrations
Surfaces with Controlled Wettability to Identify Substances at Ultra-low concentrations
An international group of physicists from Far Eastern Federal University (FEFU), the Russian Academy of Sciences, and Swinburne University of Technology (Australia) developed a technology for trapping and chemical analysis of organic and non-organic molecules at ultra low concentrations. The article was featured in the Nanoscale journal issued under the patronage of the Royal Society of Chemistry.
The discovery leads to more productive solutions in microbiology, medicine, chemistry, and biochemistry by offering quick identification of dangerous and toxic substances, cancer markers, and metabolites of pathogenic microorganisms in trace concentrations.
Using Teflon as a substrate the team created a special concentrator platform, which enables a millionfold increase in concentration of the molecules being identified. The technology cuts down the time required for advanced biochemical analyses from several days to just a few hours.
A trap for target molecules
"The key element of the concentrator is a micro- and nano-structured superhydrophilic (water attracting) trap surrounded by a superhydrophobic (water repellent) area," explains Alexey Zhizhchenko, a research associate of the Nanotechnology Research and Education Center, School of Engineering, FEFU. "In our concentrator an ordinary liquid droplet plays the role of a container for controlled transport of the target molecules to the superhydrophilic trap. This transport is made possible by controlling the size and position of the droplet in the course of vaporization, which is achieved by tailoring and optimizing the wetting properties of the substrate. Up to 97% of the target molecules are then localized on the small trap (of the size of a human hair thickness) and their concentration increases more than a million times compared to its initial value. This leads to a dramatic enhancement of the optical response of the trapped molecules. By combining this feature with highly sensitive chemical detection properties of the trap, scientists are able to detect and identify target substances even if there are just a few hundred molecules of those in the droplet.
Moreover, with further optimization of the concentrator design on can potentially achieve single molecule detection.
New cheaper ways for identifying trace concetrations of substances
The concentrator platform is fabricated by direct laser recording on Teflon substrates with ultrashort pulses. The process does not require tight laser focusing and therefore takes only a couple of minutes to complete. The technology is versatile, relatively cheap, and can be potentially used for making new generations of biosensor platforms for highly accurate and sensitive chemical analysis.
"This achievement by the team of the Nanotechnology Research and Education Center (School of Engineering, FEFU) became possible thanks to the "Materials" priority project which is currently being implemented at FEFU. We managed to gather an active international team of world-level scientists, many of whom are under 30 years old," commented Kirill Golokhvast, the Vice Rector for Research at FEFU.
Earlier this year the same research team from FEFU as a part of an international workgroup of scientists developed a technology for identifying trace concentrations of substances using a black silicon substrate platform. Due to its specific morphology black silicon amplifies the Raman signal - the light scattered by the molecules being analyzed - and does not distort spectroscopic results due to its noninvasiveness, which means that it does not react with the substance in question.