Refractive index detection at the next level
The detector is the “eye” of an HPLC system. Without this crucial component no conclusions can be drawn from the previous separation. That’s why choosing the right detection method is important for the success of an HPLC analysis.
Over the last decades a large variety of detection methods were established in the market like UV/Vis, fluorescence, light scattering, mass spectrometry (MS), or refractive index (RI). Even if MS detectors have become more important and more affordable over the last years, their price tag is still many times that of a classical UV or RI detector. UV detectors are very common, quite sensitive and easy to handle, but detection always requires the analytes to feature a decent chromophore. This is where refractive index detection comes into play, because it can detect compounds with no or just little UV activity like alcohols, sugars, amino acids or lipids.
Modern RI detectors still play an important role in today’s LC systems as a very reliable unspecific detector and there have also been improvements with RI detectors. The Azura RID 2.1L from Knauer is a good example for a well-established detection principle, implemented with modern state of the art technology. Compared to the company’s previous generation of refractive index detectors the Azura RID 2.1L comes with up to three times better sensitivity. The fully redesigned optical unit with advanced temperature control from 30 to 55 °C ensures high baseline stability, a major issue of former generations of refractive index detectors. A baseline drift of only 200 nRIU/h and a noise level of ± 2.5 nRIU are outstanding technical parameters. With its maximum flow rate of 10 ml/min and a linearity greater than 1000 µRIU the Azura RID 2.1L is the ideal detector for analytical and semi-preparative HPLC as well as for GPC applications. All common LC solvents can be used with this RI detector since it covers a large refractive index range from 1.00 RIU up to 1.75 RIU. Thus, even high RI solvents like toluene or xylenes can be used. The comparatively pressure-resistant flow cell, the LED light source with a lifetime of 20000 hours and the diagnostic functions guarantee easy handling and keep maintenance to a minimum.
In case of highly concentrated samples or compounds causing large refractive index differences, the AZURA RID 2.1L comes with the advanced feature of an extended dynamic range. This option enables the extension of the dynamic measuring range to +100 % (-1000 μRIU offset) or -100 % (+1000 μRIU offset) and broadens the range of application areas.
The Azura RID 2.1L supports the control software packages OpenLAB EZChrom Edition, ClarityChrom, Chromeleon, and PurityChrom, allowing easy integration into existing HPLC systems. With the Knauer Mobile Control, a Windows 10 based app, and its 2.5 V analog-out connector, it can also be used as a stand-alone detector for various applications.
If you require more Information please visit Knauer here.
If you want to know more about the new Azura refractive index detector or the range of other detection systems please contact Knauer here.