Biofuel Technology: Protein, Lignin and Cellulose Recovery from Ionic Liquid-Dissolved Biomass
Biofuel technology: We investigated the use of 1-ethyl-3-methylimidazonium acetate (EmimAc) to dissolve soybean mean and soybean flakes. The effect of temperature over the range of 40-70 °C was studied. An initial isolation technique precipitated all of the dissolved cellulose and some protein.
The amount of protein that co-precipitated with cellulose was dependent on the applied dissolution temperature. Acid precipitation (50 % HCl) was used to recover the protein from solution using a drop-and-stir technique. Cellulose-protein pellets were separated with either 30 % NaOH, guanidine HCl, or 8M urea causing the protein component to re-dissolve into solution and the cellulose to remain in its crystalline form.
The lignin component of the dissolved biomass was isolated with a technique which involves the use of a mixture of methyl-isobutylketone (MIBK) and ethanol. FTIR analysis was used to analyze the recovered cellulose, protein, and lignin. SDS-PAGE was used to fractionate the recovered protein before performing a MALDI-TOF MS analysis.
This poster was presented at Pittcon 2011, 13 - 18 March in Atlanta, GA, USA.
Victor Essel* and Douglas Raynie
Department of Chemistry and Biochemistry
South Dakota State University
Brookings, SD 57007 USA