Cheat Sheet: Plasmid Isolation
Plasmids usually occur in prokaryotes as closed-circle, extra-chromosomal dsDNA molecules. In molecular biology they are used for the
duplication and analysis of DNA sequences. This standard protocol is suitable for the purification of plasmids from Escherichia-coli cells. The yield
depends on the plasmid and bacterial strain used.
In the G.I.T. Laboratory CheatSheet "Plasmid Isolation" you find a description of this standard protocol including the following aspects:
- The Principle
- Take Note!