Next Generation Sequencing and its Applications
Tag-based Next Generation Sequencing: Tag-based sequencing approaches, originally designed to increase the throughput of capillary sequencing, developed into fundamentally new technologies by combination with Next Generation Sequencing (NGS). NGS allows for economic large scale sequencing projects in research and medicine. Today, Tag-based sequencing approaches and methods derived thereof are in particular suitable for analytical applications in transcript and genome analysis, where the focus shifts from 'sequencing power' to a wider range of applications, dedicated data analysis and data visualization for common users. Matthias Harbers' and Günter Kahl's comprehensive book on the topic gives an overview on recently developed NGS applications, along with means of their data analysis and experimental design. It follows a real hands-on approach with protocols and user guides and is a perfect entry for scientists to the most common and important NGS experiments.
o What was the reason to write this book?
Authors: High speed sequencing methods have rapidly changed life science and medical research. The possible applications by far exceed the commonly known genome sequencing. Therefore we intended to introduce a wide range of sequencing based technologies with our book. The book provides protocols and advice from experienced researchers in the field to guide beginners on how to conduct their own experiments using these important methods
o What is the target audience for this book?
Authors: Researchers in the medical and life science field, who are considering the use of sequencing based methods for their research.
o Which background information does the reader need?
Authors: Basic understanding of molecular biology technologies
o What is the structure of the book?
Authors: The book comprises three parts covering applications, sequencing methods, and data analysis. It provides protocols and guidance on how to conduct different analytical sequencing experiments.
Matthias Harbers graduated from the University of Hamburg where he worked on tumor development.
At the Karolinska Institute, Sweden, and in Strasbourg, France, he later studied nuclear receptors. As a visiting scientist to the RIKEN Center for Life Science Technology, he is a member of the FANTOM Project and contributed to the development of Cap-Analysis Gene Expression (CAGE) for genome-wide mapping of transcription start sites by deep sequencing.
Günter Kahl is Professor of Plant Molecular Biology, Molecular BioSciences at Frankfurt University (Germany) and presently focuses on genome analyses, fungal pathogen genome sequencing, bacterial, plant and human gene expression profiling, and the development of novel technologies. He is Co-founder and Chief Scientific Officer at SME GenXPro, Frankfurt Innovation Center Biotechnology.
1. Edition December 2011
582 Pages, Hardcover
117 Fig. (54 Colored Fig.), 19 Tab.
Authors: Matthias Harbers & Günther Kahl
Also available as ebook
Free sample chapter: