GPC/SEC/GFC: How to Select the Best Stationary Phase
Practical Advice for Method Development and Optimization
- GPC/SEC/GFC: How to Select the Best Stationary Phase
In this video clip, the “Magic Triangle” for the selection of stationary phases for GPC/SEC/GFC is presented by Dr. Daniela Held (Director Marketing and Sales, PSS). This tool provides a quick visual aid for the selection of columns, where the three sides represent sample, mobile phase, and stationary phase.
How to find the perfect GPC/SEC/GFC Column
GPC/SEC/GFC is a well-established liquid chromatography (LC) separation technique for the analysis of all soluble macromolecules (synthetic and biological). There is a large variety of GPC/SEC/ GFC columns available on the market and like in all LC techniques the choice of a column is the key to good results. Columns come in different dimensions, e.g. as preparative, analytical and micro-bore columns, and are packed with different types of stationary phases. Those stationary phases vary with respect to particle size and are offered with different sized pores. Finding the perfect column(s) to obtain long-term reproducible results can be quite challenging.
This webinar discusses basic concepts for selecting GPC/SEC/GFC columns with respect to analytical tasks, resolution and long-term reproducibility.
Practical advice for method development as well as optimization for robust, balanced GPC/SEC/GFC methods will be given, in addition to guidelines on how to minimize interaction in GPC/SEC/GFC.
Key Learning Objectives:
- How to select the best stationary phase
- Understanding the influence of particle size and pore size on resolution
- How to test columns
- How to establish a proper method
- How to establish faster GPC